Description

HPLC analysis focuses on macromolecule isolation through chemical interaction, affinity or hydrodynamic volume. SEC-HPLC works by physical interaction with the chromatography columns porous media – this is a noteworthy difference between SEC and many other liquid chromatography techniques. Chemical interaction of the sample with the column is not required or wanted as the separation should be based only on the molecular size (by a particle’s Stokes radius). SEC is used primarily for the analysis of large molecules such as proteins, polymers and polysaccharides.